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pET45 His-proSAAS (21 kDa)
(Plasmid #231636)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET45(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use strain BL21 for protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pcsk1N
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Pcsk1n (a.k.a. KEP, PEN, PEN19, PEN20, Pan3, SAAS, SAASCT, bLEN, lLEN, proSAAS)
  • Tag / Fusion Protein
    • 6x His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET45 His-proSAAS (21 kDa) was a gift from Iris Lindberg (Addgene plasmid # 231636 ; http://n2t.net/addgene:231636 ; RRID:Addgene_231636)

  • For your References section:

    A novel function for proSAAS as an amyloid anti-aggregant in Alzheimer's disease. Hoshino A, Helwig M, Rezaei S, Berridge C, Eriksen JL, Lindberg I. J Neurochem. 2014 Feb;128(3):419-30. doi: 10.1111/jnc.12454. Epub 2013 Oct 24. 10.1111/jnc.12454 PubMed 24102330
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