Flag-mproSAAS (27 kDa)
(Plasmid
#231635)
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PurposeMammalian expression of a flag-tagged full-length version of the mouse chaperone protein proSAAS.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 (-)
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePcsk1N
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SpeciesM. musculus (mouse)
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Entrez GenePcsk1n (a.k.a. KEP, PEN, PEN19, PEN20, Pan3, SAAS, SAASCT, bLEN, lLEN, proSAAS)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag-mproSAAS (27 kDa) was a gift from Iris Lindberg (Addgene plasmid # 231635 ; http://n2t.net/addgene:231635 ; RRID:Addgene_231635) -
For your References section:
Sequestration of TDP-43(216-414) Aggregates by Cytoplasmic Expression of the proSAAS Chaperone. Peinado JR, Chaplot K, Jarvela TS, Barbieri EM, Shorter J, Lindberg I. ACS Chem Neurosci. 2022 Jun 1;13(11):1651-1665. doi: 10.1021/acschemneuro.2c00156. Epub 2022 May 12. 10.1021/acschemneuro.2c00156 PubMed 35549000