Skip to main content
Addgene

Flag-mproSAAS (27 kDa)
(Plasmid #231635)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231635 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1 (-)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pcsk1N
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Pcsk1n (a.k.a. KEP, PEN, PEN19, PEN20, Pan3, SAAS, SAASCT, bLEN, lLEN, proSAAS)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-mproSAAS (27 kDa) was a gift from Iris Lindberg (Addgene plasmid # 231635 ; http://n2t.net/addgene:231635 ; RRID:Addgene_231635)
  • For your References section:

    Sequestration of TDP-43(216-414) Aggregates by Cytoplasmic Expression of the proSAAS Chaperone. Peinado JR, Chaplot K, Jarvela TS, Barbieri EM, Shorter J, Lindberg I. ACS Chem Neurosci. 2022 Jun 1;13(11):1651-1665. doi: 10.1021/acschemneuro.2c00156. Epub 2022 May 12. 10.1021/acschemneuro.2c00156 PubMed 35549000