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Addgene

mproSAAS (21 kDa)
(Plasmid #231634)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231634 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pcsk1N
  • Species
    M. musculus (mouse)
  • Mutation
    Deletion of C-terminus (aa181-225)
  • Entrez Gene
    Pcsk1n (a.k.a. KEP, PEN, PEN19, PEN20, Pan3, SAAS, SAASCT, bLEN, lLEN, proSAAS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains the L83Q polymorphism that was in the original mPcsk1n consensus sequence. This SNP is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mproSAAS (21 kDa) was a gift from Iris Lindberg (Addgene plasmid # 231634 ; http://n2t.net/addgene:231634 ; RRID:Addgene_231634)

  • For your References section:

    Functional characterization of ProSAAS: similarities and differences with 7B2. Fortenberry Y, Hwang JR, Apletalina EV, Lindberg I. J Biol Chem. 2002 Feb 15;277(7):5175-86. doi: 10.1074/jbc.M104531200. Epub 2001 Nov 21. 10.1074/jbc.M104531200 PubMed 11719503
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