pVCS5
(Plasmid
#231619)
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PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS2 at R2 site.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA231
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Backbone manufacturerSEVA Collection
- Backbone size w/o insert (bp) 2996
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePcym-VanR-mKate2-Pvan-CymR-sfGFP
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SpeciesSynthetic
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Insert Size (bp)3618
- Promoter Pvan/Pcym
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.20.604438 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVCS5 was a gift from Hans Christopher Bernstein (Addgene plasmid # 231619 ; http://n2t.net/addgene:231619 ; RRID:Addgene_231619) -
For your References section:
Fine Tuning Genetic Circuits via Host Context and RBS Modulation. Chan DTC, Winter L, Bjerg J, Krsmanovic S, Baldwin GS, Bernstein HC. bioRxiv 2024.07.20.604438 10.1101/2024.07.20.604438