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Addgene

pVCS5
(Plasmid #231619)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231619 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSEVA231
  • Backbone manufacturer
    SEVA Collection
  • Backbone size w/o insert (bp) 2996
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    3618
  • Promoter Pvan/Pcym

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.20.604438 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVCS5 was a gift from Hans Christopher Bernstein (Addgene plasmid # 231619 ; http://n2t.net/addgene:231619 ; RRID:Addgene_231619)
  • For your References section:

    Fine Tuning Genetic Circuits via Host Context and RBS Modulation. Chan DTC, Winter L, Bjerg J, Krsmanovic S, Baldwin GS, Bernstein HC. bioRxiv 2024.07.20.604438 10.1101/2024.07.20.604438