pS5
(Plasmid
#231614)
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PurposeL-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signals
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA231
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Backbone manufacturerSEVA Collection
- Backbone size w/o insert (bp) 2996
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePbad-TetR-sfGFP-Ptet-AraC-mKate2
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SpeciesSynthetic
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Insert Size (bp)3989
- Promoter Pbad/Ptet
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pS5 was a gift from Hans Christopher Bernstein (Addgene plasmid # 231614 ; http://n2t.net/addgene:231614 ; RRID:Addgene_231614) -
For your References section:
Pangenomic landscapes shape performances of a synthetic genetic circuit across Stutzerimonas species. Chan DTC, Bernstein HC. mSystems. 2024 Sep 17;9(9):e0084924. doi: 10.1128/msystems.00849-24. Epub 2024 Aug 21. 10.1128/msystems.00849-24 PubMed 39166875