pS4
(Plasmid
#231613)
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PurposeL-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signals
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA231
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Backbone manufacturerSEVA Collection
- Backbone size w/o insert (bp) 2996
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePtet-AraC-mKate2-Pbad-TetR-sfGFP
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SpeciesSynthetic
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Insert Size (bp)3970
- Promoter Pbad/Ptet
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pS4 was a gift from Hans Christopher Bernstein (Addgene plasmid # 231613 ; http://n2t.net/addgene:231613 ; RRID:Addgene_231613) -
For your References section:
Revealing the Host-Dependent Nature of an Engineered Genetic Inverter in Concordance with Physiology. Chan DTC, Baldwin GS, Bernstein HC. Biodes Res. 2023 Aug 16;5:0016. doi: 10.34133/bdr.0016. eCollection 2023. 10.34133/bdr.0016 PubMed 37849456