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Addgene

pGEM-7Zf-wild
(Plasmid #23160)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23160 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-7Zf-wild
  • Backbone size w/o insert (bp) 2997
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ribonuclease assay sub1
  • Insert Size (bp)
    188

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AatII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a small gap in alignment between depositor's sequence and Addgene's sequence with T7 primer.
The discrepancy is due to a very stable stem loop, which makes it difficult to do DNA sequencing without cutting it with a restriction enzyme. You need to cut it at a PstI site in the loop region before doing DNA sequencing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-7Zf-wild was a gift from SeWon Suh (Addgene plasmid # 23160 ; http://n2t.net/addgene:23160 ; RRID:Addgene_23160)

  • For your References section:

    Structural and functional insights into dom34, a key component of no-go mRNA decay. Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park CM, Suh SW. Mol Cell. 2007 Sep 21. 27(6):938-50. 10.1016/j.molcel.2007.07.019 PubMed 17889667
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