pHR-scFv-GCN4-mStayGold-GB1-dWPRE
(Plasmid
#231598)
-
PurposeExpress scFv_GCN4-mStayGold for SunTag translation imaging
-
Depositing Lab
-
Sequence Information
-
Sequences (1) — Accept Affinity Reagent Sequence Policy
-
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 231598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHR
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namescFv-GCN4-mStayGold
-
SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHR-scFv-GCN4-mStayGold-GB1-dWPRE was a gift from Marvin Tanenbaum (Addgene plasmid # 231598 ; http://n2t.net/addgene:231598 ; RRID:Addgene_231598) -
For your References section:
Long-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translation. Madern MF, Yang S, Witteveen O, Segeren HA, Bauer M, Tanenbaum ME. Cell. 2025 Jan 23:S0092-8674(25)00045-5. doi: 10.1016/j.cell.2025.01.016. 10.1016/j.cell.2025.01.016 PubMed 39892379