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Addgene

pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-2xXbp1(S255A)
(Plasmid #231596)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Kozak sequence-Tornado-2xALFATag-10xSunTag-2xXbp1(S255A) pause sequence
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-2xXbp1(S255A) was a gift from Marvin Tanenbaum (Addgene plasmid # 231596 ; http://n2t.net/addgene:231596 ; RRID:Addgene_231596)

  • For your References section:

    Long-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translation. Madern MF, Yang S, Witteveen O, Segeren HA, Bauer M, Tanenbaum ME. Cell. 2025 Jan 23:S0092-8674(25)00045-5. doi: 10.1016/j.cell.2025.01.016. 10.1016/j.cell.2025.01.016 PubMed 39892379
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