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Addgene

pInducer20 ALPK1 WT
(Plasmid #231585)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231585 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pINDUCER20
  • Backbone manufacturer
    Addgene 44012
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ALPK1
  • Alt name
    alpha kinase 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3735
  • Entrez Gene
    ALPK1 (a.k.a. 8430410J10Rik, LAK, ROSAH)
  • Promoter doxycycline
  • Tag / Fusion Protein
    • 3X flag (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer GTCCAGTGTGGTGGaATTC
  • 3′ sequencing primer GCCACTGTGCTGGATATCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pInducer20 ALPK1 WT was a gift from Thomas Henry (Addgene plasmid # 231585 ; http://n2t.net/addgene:231585 ; RRID:Addgene_231585)

  • For your References section:

    IFN-gamma licenses normal and pathogenic ALPK1/TIFA pathway in human monocytes. Martin A, Caron S, Marcotte M, Bronnec P, Garneret E, Martel N, Maalouf G, Seve P, Saadoun D, Jamilloux Y, Henry T. iScience. 2024 Dec 10;28(1):111563. doi: 10.1016/j.isci.2024.111563. eCollection 2025 Jan 17. 10.1016/j.isci.2024.111563 PubMed 39868044
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