Skip to main content
Addgene

TRE3G-PE2-P2A-BFP
(Plasmid #231580)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231580 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    LentiCRISPRv2
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2-P2A-BFP
  • Alt name
    Cas9(H840A)-M-MLVrt(D200N, T306K, W313F, T330P, L603W)
  • Species
    Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
  • Insert Size (bp)
    7059
  • Promoter TRE3G
  • Tag / Fusion Protein
    • P2A-BFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRE3G-PE2-P2A-BFP was a gift from Jesse Engreitz (Addgene plasmid # 231580 ; http://n2t.net/addgene:231580 ; RRID:Addgene_231580)

  • For your References section:

    Rewriting regulatory DNA to dissect and reprogram gene expression. Martyn GE, Montgomery MT, Jones H, Guo K, Doughty BR, Linder J, Chen Z, Cochran K, Lawrence KA, Munson G, Pampari A, Fulco CP, Kelley DR, Lander ES, Kundaje A, Engreitz JM. bioRxiv [Preprint]. 2023 Dec 21:2023.12.20.572268. doi: 10.1101/2023.12.20.572268. 10.1101/2023.12.20.572268 PubMed 38187584
Related items: