Tip alpha (28-192)
(Plasmid
#23158)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28b(+)
- Backbone size w/o insert (bp) 5368
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTNF-α-inducing protein
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Alt nameTipα
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SpeciesHelicobacter pylori
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Insert Size (bp)495
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Mutationdeleted amino acids 1-27
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GenBank IDNP_223261
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tip alpha (28-192) was a gift from SeWon Suh (Addgene plasmid # 23158 ; http://n2t.net/addgene:23158 ; RRID:Addgene_23158) -
For your References section:
Crystal structure of the TNF-alpha-Inducing protein (Tipalpha) from Helicobacter pylori: Insights into Its DNA-binding activity. Jang JY, Yoon HJ, Yoon JY, Kim HS, Lee SJ, Kim KH, Kim do J, Jang S, Han BG, Lee BI, Suh SW. J Mol Biol. 2009 Sep 11. 392(1):191-7. 10.1016/j.jmb.2009.07.010 PubMed 19596016