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Addgene

dSpyCas9-E566-GS-LOV2-VPR
(Plasmid #231576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dSpyCas9-VPR with an AsLOV2 insertion behind E566
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.06.565805 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dSpyCas9-E566-GS-LOV2-VPR was a gift from Dominik Niopek (Addgene plasmid # 231576 ; http://n2t.net/addgene:231576 ; RRID:Addgene_231576)

  • For your References section:

    Rational engineering of allosteric protein switches by in silico prediction of domain insertion sites. Wolf B, Shehu P, Brenker L, von Bachmann A, Kroell A-S, Southern N, Holderbach S, Eigenmann J, Aschenbrenner S, Mathony J, Niopek D. bioRxiv 2024.12.04.626757 10.1101/2024.12.04.626757
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