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Addgene

CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3
(Plasmid #231568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    All-in-one LOOMINA construct
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.06.565805 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3 was a gift from Dominik Niopek (Addgene plasmid # 231568 ; http://n2t.net/addgene:231568 ; RRID:Addgene_231568)

  • For your References section:

    A modular toolbox for the optogenetic deactivation of transcription. Muench P, Fiumara M, Southern N, Coda D, Aschenbrenner S, Correia B, Graff J, Niopek D, Mathony J. Nucleic Acids Res. 2024 Dec 16:gkae1237. doi: 10.1093/nar/gkae1237. 10.1093/nar/gkae1237 PubMed 39676667
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