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pCMVtrunc sfCherry2ΔC4-Lifeact
(Plasmid #231557)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231557 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMVtrunc (Watanabe and Mitchison, 2002)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lifeact
  • Alt name
    first 17 residues of budding yeast Abp140
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ABP140 (a.k.a. YOR239W, TRM140, YOR240W)
  • Promoter CMVtrunc
  • Tag / Fusion Protein
    • sfCherry2 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.26.591305 for bioRxiv preprint.

This plasmid is also called L81 in the paper.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVtrunc sfCherry2ΔC4-Lifeact was a gift from Manos Mavrakis (Addgene plasmid # 231557 ; http://n2t.net/addgene:231557 ; RRID:Addgene_231557)

  • For your References section:

    Genetically encoded reporters of actin filament organization in living cells and tissues. Martins CS, Iv F, Suman SK, Panagiotou TC, Sidor C, Ruso-López M, Plancke CN, Omi S, Gomes M, Llewellyn A, Bandi SR, Ramond L, Arbizzani F, Rimoli CV, Schnorrer F, Robin F, Wilde A, LeGoff L, Pedelacq J-D, Cabantous S, Rincon SA, Chandre C, Brasselet S, Mavrakis M. bioRxiv 2024.04.26.591305 10.1101/2024.04.26.591305
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