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Addgene

pCMVtrunc Affimer6-msfGFPΔN12
(Plasmid #231550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMVtrunc (Watanabe and Mitchison, 2002)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Affimer6
  • Species
    Synthetic
  • Promoter CMVtrunc
  • Tag / Fusion Protein
    • msfGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.26.591305 for bioRxiv preprint.

This plasmid is also called Af7 in the paper.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVtrunc Affimer6-msfGFPΔN12 was a gift from Manos Mavrakis (Addgene plasmid # 231550 ; http://n2t.net/addgene:231550 ; RRID:Addgene_231550)

  • For your References section:

    Genetically encoded reporters of actin filament organization in living cells and tissues. Martins CS, Iv F, Suman SK, Panagiotou TC, Sidor C, Ruso-López M, Plancke CN, Omi S, Gomes M, Llewellyn A, Bandi SR, Ramond L, Arbizzani F, Rimoli CV, Schnorrer F, Robin F, Wilde A, LeGoff L, Pedelacq J-D, Cabantous S, Rincon SA, Chandre C, Brasselet S, Mavrakis M. bioRxiv 2024.04.26.591305 10.1101/2024.04.26.591305
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