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Addgene

pBAD25
(Plasmid #231547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231547 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Modifications to backbone
    Exchanged AmpR gene with KanR gene from pKD4 vector with newly added restriction sites NdeI and SacI.
  • Vector type
    Bacterial Expression
  • Promoter pBAD

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD25 was a gift from Martin Wiedmann (Addgene plasmid # 231547 ; http://n2t.net/addgene:231547 ; RRID:Addgene_231547)

  • For your References section:

    Engineering of a monitorable expression system to characterize beta-lactamase genes in Enterobacteriaceae. Schumann A, Gaballa A, Wiedmann M. J Microbiol Methods. 2025 Mar 18;232-234:107120. doi: 10.1016/j.mimet.2025.107120. 10.1016/j.mimet.2025.107120 PubMed 40113096
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