pETG20a-Salro-HMG
(Plasmid
#231543)
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PurposeExpresses the HMG of Salro Sox-like, for protein purification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETG20a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe depositing lab uses Rosetta2 E. coli for protein expression and purification.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSox-Iike HMG
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SpeciesSalpingoeca rosetta
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETG20a-Salro-HMG was a gift from Ralf Jauch (Addgene plasmid # 231543 ; http://n2t.net/addgene:231543 ; RRID:Addgene_231543) -
For your References section:
The emergence of Sox and POU transcription factors predates the origins of animal stem cells. Gao Y, Tan DS, Girbig M, Hu H, Zhou X, Xie Q, Yeung SW, Lee KS, Ho SY, Cojocaru V, Yan J, Hochberg GKA, de Mendoza A, Jauch R. Nat Commun. 2024 Nov 14;15(1):9868. doi: 10.1038/s41467-024-54152-x. 10.1038/s41467-024-54152-x PubMed 39543096