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Addgene

pET28a-Salhel-HMG
(Plasmid #231539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab uses Rosetta2 E. coli for protein expression and purification.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Sox-I HMG
  • Species
    Salpingoeca rosetta
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-Salhel-HMG was a gift from Ralf Jauch (Addgene plasmid # 231539 ; http://n2t.net/addgene:231539 ; RRID:Addgene_231539)

  • For your References section:

    The emergence of Sox and POU transcription factors predates the origins of animal stem cells. Gao Y, Tan DS, Girbig M, Hu H, Zhou X, Xie Q, Yeung SW, Lee KS, Ho SY, Cojocaru V, Yan J, Hochberg GKA, de Mendoza A, Jauch R. Nat Commun. 2024 Nov 14;15(1):9868. doi: 10.1038/s41467-024-54152-x. 10.1038/s41467-024-54152-x PubMed 39543096
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