pHAGE2-TetO-Chim-Pchi-Q61A/E64M
(Plasmid
#231521)
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PurposeExpresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231521 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE2-TetO
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePchi HMG Q61A/E64M flanked with N- and C-termini of mSox2
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SpeciesPigoraptor chileana
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MutationPoint mutations at position 61 Q to A and 64 E to M of the HMG domain
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE2-TetO-Chim-Pchi-Q61A/E64M was a gift from Ralf Jauch (Addgene plasmid # 231521 ; http://n2t.net/addgene:231521 ; RRID:Addgene_231521) -
For your References section:
The emergence of Sox and POU transcription factors predates the origins of animal stem cells. Gao Y, Tan DS, Girbig M, Hu H, Zhou X, Xie Q, Yeung SW, Lee KS, Ho SY, Cojocaru V, Yan J, Hochberg GKA, de Mendoza A, Jauch R. Nat Commun. 2024 Nov 14;15(1):9868. doi: 10.1038/s41467-024-54152-x. 10.1038/s41467-024-54152-x PubMed 39543096