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pSEVA47_dCas9-GFP_YES_i2
(Plasmid #231419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231419 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA471
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10 ; TOP10
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    dCas9
  • Species
    Synthetic

Gene/Insert 2

  • Gene/Insert name
    GFP
  • Species
    Synthetic

Gene/Insert 3

  • Gene/Insert name
    sgRNA-Y
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA47_dCas9-GFP_YES_i2 was a gift from Manish Kushwaha (Addgene plasmid # 231419 ; http://n2t.net/addgene:231419 ; RRID:Addgene_231419)

  • For your References section:

    Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia. Pujar A, Pathania A, Hopper C, Pandi A, Calderon CR, Fugger M, Nowak T, Kushwaha M. Nucleic Acids Res. 2024 Dec 27:gkae1256. doi: 10.1093/nar/gkae1256. 10.1093/nar/gkae1256 PubMed 39727169
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