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pSEVA47_dCas9-GFP_NOT_iA
(Plasmid #231417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231417 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA471
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10 ; TOP10
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    dCas9
  • Species
    Synthetic

Gene/Insert 2

  • Gene/Insert name
    GFP
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please also refer to Pujar et al. 2024 Nucleic Acids Res. (https://doi.org/10.1093/nar/gkae1256, and see https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint) for additional work using this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA47_dCas9-GFP_NOT_iA was a gift from Alfonso Jaramillo (Addgene plasmid # 231417 ; http://n2t.net/addgene:231417 ; RRID:Addgene_231417)

  • For your References section:

    Development of a genetically-encoded in vivo computing platform to play the game of Tic-Tac-Toe. Kushwaha M, Singh V, Jaramillo A. Zenodo (2017) Conference: SB7.0: The Seventh International Meeting on Synthetic Biology. 10.5281/zenodo.14580985
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