pSEVA26_sgRNA-A0_M13ps
(Plasmid
#231413)
-
PurposeM13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 231413 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSEVA261
-
Vector typeSynthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10 ; TOP10
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namesgRNA-A0
-
SpeciesSynthetic
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSEVA26_sgRNA-A0_M13ps was a gift from Manish Kushwaha (Addgene plasmid # 231413 ; http://n2t.net/addgene:231413 ; RRID:Addgene_231413) -
For your References section:
Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia. Pujar A, Pathania A, Hopper C, Pandi A, Calderon CR, Fugger M, Nowak T, Kushwaha M. Nucleic Acids Res. 2024 Dec 27:gkae1256. doi: 10.1093/nar/gkae1256. 10.1093/nar/gkae1256 PubMed 39727169