pSEVA19_sgRNA-C0_M13ps
(Plasmid
#231410)
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PurposeM13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA191
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA-C0
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please also refer to Pujar et al. 2024 Nucleic Acids Res. (https://doi.org/10.1093/nar/gkae1256, and see https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint) for additional work using this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEVA19_sgRNA-C0_M13ps was a gift from Alfonso Jaramillo (Addgene plasmid # 231410 ; http://n2t.net/addgene:231410 ; RRID:Addgene_231410) -
For your References section:
Development of a genetically-encoded in vivo computing platform to play the game of Tic-Tac-Toe. Kushwaha M, Singh V, Jaramillo A. Zenodo (2017) Conference: SB7.0: The Seventh International Meeting on Synthetic Biology. 10.5281/zenodo.14580985