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Addgene

pAGC-Lb12-P1Bb
(Plasmid #231387)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231387 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC57
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6-29
  • Alt name
    pAGC-8xLb-P1
  • gRNA/shRNA sequence
    U6-29 cassette
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGC-Lb12-P1Bb was a gift from Qi-Jun Chen (Addgene plasmid # 231387 ; http://n2t.net/addgene:231387 ; RRID:Addgene_231387)
  • For your References section:

    Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations. Xin C, Qiao D, Wang J, Sun W, Cao Z, Lu Y, Jiang Y, Chai Y, Wang XC, Chen QJ. aBIOTECH. 2024 Mar 26;5(2):117-126. doi: 10.1007/s42994-024-00144-w. eCollection 2024 Jun. 10.1007/s42994-024-00144-w PubMed 38978783