pAGC-Lb12-P1Bb
(Plasmid
#231387)
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PurposeFor crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231387 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameU6-29
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Alt namepAGC-8xLb-P1
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gRNA/shRNA sequenceU6-29 cassette
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SpeciesSynthetic
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAGC-Lb12-P1Bb was a gift from Qi-Jun Chen (Addgene plasmid # 231387 ; http://n2t.net/addgene:231387 ; RRID:Addgene_231387) -
For your References section:
Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations. Xin C, Qiao D, Wang J, Sun W, Cao Z, Lu Y, Jiang Y, Chai Y, Wang XC, Chen QJ. aBIOTECH. 2024 Mar 26;5(2):117-126. doi: 10.1007/s42994-024-00144-w. eCollection 2024 Jun. 10.1007/s42994-024-00144-w PubMed 38978783