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Addgene

pBG-ttLbUV2
(Plasmid #231386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCambia
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ttLbCas12a Ultra V2
  • gRNA/shRNA sequence
    U6-crRNA-HDV-U6t cassette
  • Species
    Synthetic
  • Mutation
    D156R and E795L

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBG-ttLbUV2 was a gift from Qi-Jun Chen (Addgene plasmid # 231386 ; http://n2t.net/addgene:231386 ; RRID:Addgene_231386)

  • For your References section:

    Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations. Xin C, Qiao D, Wang J, Sun W, Cao Z, Lu Y, Jiang Y, Chai Y, Wang XC, Chen QJ. aBIOTECH. 2024 Mar 26;5(2):117-126. doi: 10.1007/s42994-024-00144-w. eCollection 2024 Jun. 10.1007/s42994-024-00144-w PubMed 38978783
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