Skip to main content
Addgene

pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R635A)
(Plasmid #231384)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231384 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.3
  • Backbone size w/o insert (bp) 5413
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human Argonaute2
  • Alt name
    AGO2
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2958
  • Mutation
    R635A
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG-SUMO_Eu1 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

With engineered orthogonal SUMO_Eu1 (Vera Rodriguez et al., JCB 2019) in N-terminal tag.

Please visit https://doi.org/10.1101/2024.08.19.608718 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R635A) was a gift from David Bartel (Addgene plasmid # 231384 ; http://n2t.net/addgene:231384 ; RRID:Addgene_231384)

  • For your References section:

    The structural basis for RNA slicing by human Argonaute2. Mohamed AA, Wang PY, Bartel DP, Vos SM. Cell Reports (2025), Volume 44, Issue 1, 115166 10.1016/j.celrep.2024.115166
Related items: