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pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7
(Plasmid #231353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231353 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T7-SP6-BC(p11-14)
  • Species
    Other
  • Insert Size (bp)
    202

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also contains an minBG-driven mRuby2, with chimeric intron to increase expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7 was a gift from Viviana Gradinaru (Addgene plasmid # 231353 ; http://n2t.net/addgene:231353 ; RRID:Addgene_231353)

  • For your References section:

    Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo. Coughlin GM, Borsos M, Barcelona BH, Appling N, Mayfield AMH, Mackey ED, Eser RA, Jackson CR, Chen X, Kumar SR, Gradinaru V. Nat Biotechnol. 2025 Mar 20. doi: 10.1038/s41587-025-02565-4. 10.1038/s41587-025-02565-4 PubMed 40113953
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