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pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2)
(Plasmid #231234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2-GFP-IRES-mCherry
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MYC
  • Alt name
    c-myc 2
  • Alt name
    pCS2-MYC(Δ1-143; K355E; R356E; R357E; Y402A; I403A; L404A; S405A; V406A; L434E; C438E)-GFP-IRES-mCherry
  • Alt name
    ΔTADΔDeg1ΔDeg2
  • Species
    H. sapiens (human)
  • Mutation
    TAD deleted (Δ1-143), deletion of degron 1 & 2 (K355E; R356E; R357E; Y402A; I403A; L404A; S405A; V406A; L434E; C438E); numbering based on sequence for c-myc 2
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Myc numbering is based on sequence for c-myc 2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2) was a gift from Michael Rape (Addgene plasmid # 231234 ; http://n2t.net/addgene:231234 ; RRID:Addgene_231234)

  • For your References section:

    Orphan quality control shapes network dynamics and gene expression. Mark KG, Kolla S, Aguirre JD, Garshott DM, Schmitt S, Haakonsen DL, Xu C, Kater L, Kempf G, Martinez-Gonzalez B, Akopian D, See SK, Thoma NH, Rape M. Cell. 2023 Aug 3;186(16):3460-3475.e23. doi: 10.1016/j.cell.2023.06.015. Epub 2023 Jul 20. 10.1016/j.cell.2023.06.015 PubMed 37478862
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