pCS2-MCRS1-GFP-IRES-mCherry
(Plasmid
#231231)
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PurposeMCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231231 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2-GFP-IRES-mCherry
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCRS1
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SpeciesH. sapiens (human)
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Entrez GeneMCRS1 (a.k.a. ICP22BP, INO80Q, MCRS2, MSP58, P78)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-MCRS1-GFP-IRES-mCherry was a gift from Michael Rape (Addgene plasmid # 231231 ; http://n2t.net/addgene:231231 ; RRID:Addgene_231231) -
For your References section:
Orphan quality control shapes network dynamics and gene expression. Mark KG, Kolla S, Aguirre JD, Garshott DM, Schmitt S, Haakonsen DL, Xu C, Kater L, Kempf G, Martinez-Gonzalez B, Akopian D, See SK, Thoma NH, Rape M. Cell. 2023 Aug 3;186(16):3460-3475.e23. doi: 10.1016/j.cell.2023.06.015. Epub 2023 Jul 20. 10.1016/j.cell.2023.06.015 PubMed 37478862