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CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2
(Plasmid #231207)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231207 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry
  • Species
    H. sapiens (human)
  • Mutation
    L147I, R148H, I203V, L290V
  • Entrez Gene
    IL10 (a.k.a. CSIF, GVHDS, IL-10, IL10A, TGIF)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2 was a gift from Xiaojing Gao (Addgene plasmid # 231207 ; http://n2t.net/addgene:231207 ; RRID:Addgene_231207)

  • For your References section:

    Orthogonalized human protease control of secreted signals. Aldrete CA, Call CC, Sant’Anna LE, Vlahos AE, Pei J, Cong Q, Gao XJ. Nat Chem Biol (2025). 10.1038/s41589-024-01831-x
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