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pcDNA3.1-ER-mRFP
(Plasmid #231176)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231176 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5341
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ER-mRFP
  • Species
    Synthetic
  • Insert Size (bp)
    960
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthesis

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-ER-mRFP was a gift from Dmitry Lim (Addgene plasmid # 231176 ; http://n2t.net/addgene:231176 ; RRID:Addgene_231176)

  • For your References section:

    ER-mitochondria distance is a critical parameter for efficient mitochondrial Ca(2+) uptake and oxidative metabolism. Dematteis G, Tapella L, Casali C, Talmon M, Tonelli E, Reano S, Ariotti A, Pessolano E, Malecka J, Chrostek G, Kulkoviene G, Umbrasas D, Distasi C, Grilli M, Ladds G, Filigheddu N, Fresu LG, Mikoshiba K, Matute C, Ramos-Gonzalez P, Jekabsone A, Cali T, Brini M, Biggiogera M, Cavaliere F, Miggiano R, Genazzani AA, Lim D. Commun Biol. 2024 Oct 10;7(1):1294. doi: 10.1038/s42003-024-06933-9. 10.1038/s42003-024-06933-9 PubMed 39390051
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