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Addgene

pRDB_270
(Plasmid #231111)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231111 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRDA_722
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TadA-CDd-nCas9-SpG-UGI-HA
  • Species
    Synthetic
  • Mutation
    D10A

Cloning Information

  • Cloning method Golden Gate

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    David Liu and Keith Joung

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from David Liu's Base editing vectors and Keith Joung's SpG-Cas9 variant.

Please visit https://doi.org/10.1101/2024.11.12.622254 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRDB_270 was a gift from John Doench & David Root (Addgene plasmid # 231111 ; http://n2t.net/addgene:231111 ; RRID:Addgene_231111)
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