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Addgene

pMT-Ex
(Plasmid #231106)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231106 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT/BiP/V5-His C
  • Backbone manufacturer
    Invitrogen/Thermo Fisher
  • Backbone size (bp) 5484
  • Modifications to backbone
    The hygromycin resistance gene (hygromycin B phosphotransferase) added. A nucleotide was added to change the frame of the BglII restriction site with respect to the BiP signal peptide.
  • Vector type
    Insect Expression
  • Promoter Drosophila metallothionein promoter, MT
  • Selectable markers
    Hygromycin
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-Ex was a gift from Michael Jeltsch (Addgene plasmid # 231106 ; http://n2t.net/addgene:231106 ; RRID:Addgene_231106)
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