pSB19-PapRS#34-OmpA
(Plasmid
#231105)
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Purpose(Empty Backbone) Vector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231105 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB19-PapRS#34
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB19-PapRS#34-OmpA was a gift from Arne Skerra (Addgene plasmid # 231105 ; http://n2t.net/addgene:231105 ; RRID:Addgene_231105) -
For your References section:
Protein purification with light via a genetically encoded azobenzene side chain. Mayrhofer P, Anneser MR, Schira K, Sommer CA, Theobald I, Schlapschy M, Achatz S, Skerra A. Nat Commun. 2024 Dec 18;15(1):10693. doi: 10.1038/s41467-024-55212-y. 10.1038/s41467-024-55212-y PubMed 39695158