pMX222
(Plasmid #23109)

• Depositing Lab: Linda Wordeman
• Publication: Andrews et al Dev Cell. 2004 Feb . 6(2):253-68.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4709
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mitotic Centromere Associated Kinesin
• Alt name: MCAK
• Alt name: Kif2C
• Alt name: Kinesin 13
• Species: Cricetulus griseus (Chinese hamster)
• Insert Size (bp): 2238
• Mutation: S92E, S106E, S108E, S112E, S186E
• GenBank ID: U11790
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: catggtcctgctggagttcgtg
• 3′ sequencing primer: TTTTAAAGCAAGTAAAACCTCTACAAA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry