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Addgene

KPC2-pNic-Bsa4
(Plasmid #231082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-Bsa4
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    carbapenemase-2
  • Alt name
    KPC-2
  • Entrez Gene
    kpc2 (a.k.a. pCRE79_15)
  • Tag / Fusion Protein
    • 6x His tag (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TGTGTACGCGATGGATACCG
  • 3′ sequencing primer CGGCATAGTCATTTGCCGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KPC2-pNic-Bsa4 was a gift from Josh Beckham (Addgene plasmid # 231082 ; http://n2t.net/addgene:231082 ; RRID:Addgene_231082)
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