iPLAG1-3xFLAG
(Plasmid
#231081)
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PurposeExpresses PLAG1 with C-terminal 3xFLAG tag, in doxycycline-inducible manner
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepInducer20
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Backbone manufacturerplasmid 44012
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePLAG1
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SpeciesH. sapiens (human)
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Entrez GenePLAG1 (a.k.a. PSA, SGPA, SRS4, ZNF912)
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Tag
/ Fusion Protein
- 3XFLAG (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer LNCX (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
iPLAG1-3xFLAG was a gift from Kenneth Chen (Addgene plasmid # 231081 ; http://n2t.net/addgene:231081 ; RRID:Addgene_231081)