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Addgene

pYOY153
(Plasmid #23107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4709
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mitotic Centromere Associated Kinesin
  • Alt name
    MCAK
  • Alt name
    Kif2C
  • Alt name
    Kinesin 13
  • Species
    Cricetulus griseus (Chinese hamster)
  • Insert Size (bp)
    2238
  • Mutation
    H530-A, R534-A, K537-A
  • GenBank ID
    U11790
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer catggtcctgctggagttcgtg
  • 3′ sequencing primer TTTTAAAGCAAGTAAAACCTCTACAAA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYOY153 was a gift from Linda Wordeman (Addgene plasmid # 23107 ; http://n2t.net/addgene:23107 ; RRID:Addgene_23107)

  • For your References section:

    Aurora B regulates MCAK at the mitotic centromere. Andrews PD, Ovechkina Y, Morrice N, Wagenbach M, Duncan K, Wordeman L, Swedlow JR. Dev Cell. 2004 Feb . 6(2):253-68. 10.1016/S1534-5807(04)00025-5 PubMed 14960279
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