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L1p3_barley_sgRNA_scaffold
(Plasmid #231030)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231030 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH47751
  • Backbone size w/o insert (bp) 4376
  • Vector type
    Bacterial Expression, CRISPR
  • Selectable markers
    RFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Scaffold for Cas9 mediated CRISPR
  • Species
    Synthetic
  • Insert Size (bp)
    1583
  • Promoter TaU6

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer GTGGTGTAAACAAATTGACGC
  • 3′ sequencing primer CCCGCCAATATATCCTGTCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional insert mRFP.

Please visit https://doi.org/10.1101/2024.08.22.609225 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L1p3_barley_sgRNA_scaffold was a gift from Wilma van Esse (Addgene plasmid # 231030 ; http://n2t.net/addgene:231030 ; RRID:Addgene_231030)

  • For your References section:

    VRS5 (HvTB1) binds to the promoter of tillering and floral homeotic genes to regulate their expression. Winkelmolen T, Colleoni P, Moscou M, Hoseinzadeh P, Oldach K, Schmidt RC, Immink R, van Esse GW. bioRxiv 2024.08.22.609225 10.1101/2024.08.22.609225
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