L1p3_barley_sgRNA_scaffold
(Plasmid
#231030)
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PurposeCloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepICH47751
- Backbone size w/o insert (bp) 4376
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Vector typeBacterial Expression, CRISPR
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Selectable markersRFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScaffold for Cas9 mediated CRISPR
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SpeciesSynthetic
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Insert Size (bp)1583
- Promoter TaU6
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer GTGGTGTAAACAAATTGACGC
- 3′ sequencing primer CCCGCCAATATATCCTGTCA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional insert mRFP.
Please visit https://doi.org/10.1101/2024.08.22.609225 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L1p3_barley_sgRNA_scaffold was a gift from Wilma van Esse (Addgene plasmid # 231030 ; http://n2t.net/addgene:231030 ; RRID:Addgene_231030) -
For your References section:
VRS5 (HvTB1) binds to the promoter of tillering and floral homeotic genes to regulate their expression. Winkelmolen T, Colleoni P, Moscou M, Hoseinzadeh P, Oldach K, Schmidt RC, Immink R, van Esse GW. bioRxiv 2024.08.22.609225 10.1101/2024.08.22.609225