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Addgene

pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry
(Plasmid #231007)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231007 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E4F1 aa220-242
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    69
  • Entrez Gene
    E4F1 (a.k.a. E4F)
  • Tag / Fusion Protein
    • AcGFP1 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry was a gift from Michael Rape (Addgene plasmid # 231007 ; http://n2t.net/addgene:231007 ; RRID:Addgene_231007)

  • For your References section:

    Structural basis and regulation of the reductive stress response. Manford AG, Mena EL, Shih KY, Gee CL, McMinimy R, Martinez-Gonzalez B, Sherriff R, Lew B, Zoltek M, Rodriguez-Perez F, Woldesenbet M, Kuriyan J, Rape M. Cell. 2021 Oct 14;184(21):5375-5390.e16. doi: 10.1016/j.cell.2021.09.002. Epub 2021 Sep 24. 10.1016/j.cell.2021.09.002 PubMed 34562363
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