pCS2+ HA-BEX1
(Plasmid
#231002)
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PurposeTransient overexpression of human BEX1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231002 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBEX1
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SpeciesH. sapiens (human)
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Insert Size (bp)377
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Entrez GeneBEX1 (a.k.a. BEX2, HBEX2, HGR74-h)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATTTAGGTGACACTATAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+ HA-BEX1 was a gift from Michael Rape (Addgene plasmid # 231002 ; http://n2t.net/addgene:231002 ; RRID:Addgene_231002) -
For your References section:
Structural basis and regulation of the reductive stress response. Manford AG, Mena EL, Shih KY, Gee CL, McMinimy R, Martinez-Gonzalez B, Sherriff R, Lew B, Zoltek M, Rodriguez-Perez F, Woldesenbet M, Kuriyan J, Rape M. Cell. 2021 Oct 14;184(21):5375-5390.e16. doi: 10.1016/j.cell.2021.09.002. Epub 2021 Sep 24. 10.1016/j.cell.2021.09.002 PubMed 34562363