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Addgene

pGSTag BimEL (3SA)
(Plasmid #23100)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23100 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGSTag
  • Backbone size w/o insert (bp) 5057
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bim EL (3SA)
  • Alt name
    Bim
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    590
  • Mutation
    changed Serine 55/65/73 to alanine
  • Entrez Gene
    Bcl2l11 (a.k.a. 1500006F24Rik, Bim, Bod, bcl2-L-11)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGex 5'
  • 3′ sequencing primer pGex 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGSTag BimEL (3SA) was a gift from Roger Davis (Addgene plasmid # 23100 ; http://n2t.net/addgene:23100 ; RRID:Addgene_23100)

  • For your References section:

    Multisite phosphorylation regulates Bim stability and apoptotic activity. Hubner A, Barrett T, Flavell RA, Davis RJ. Mol Cell. 2008 May 23. 30(4):415-25. 10.1016/j.molcel.2008.03.025 PubMed 18498746
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