pCS2+ 3xFLAG-FEM1B WT
(Plasmid
#230993)
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PurposeTransient overexpression of mouse FEM1B in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 230993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFEM1B
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SpeciesM. musculus (mouse)
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Insert Size (bp)1184
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Entrez GeneFem1b (a.k.a. mKIAA0396)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATTTAGGTGACACTATAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+ 3xFLAG-FEM1B WT was a gift from Michael Rape (Addgene plasmid # 230993 ; http://n2t.net/addgene:230993 ; RRID:Addgene_230993) -
For your References section:
A Cellular Mechanism to Detect and Alleviate Reductive Stress. Manford AG, Rodriguez-Perez F, Shih KY, Shi Z, Berdan CA, Choe M, Titov DV, Nomura DK, Rape M. Cell. 2020 Oct 1;183(1):46-61.e21. doi: 10.1016/j.cell.2020.08.034. Epub 2020 Sep 16. 10.1016/j.cell.2020.08.034 PubMed 32941802