MLF2-GFP-3xFLAG
(Plasmid
#230986)
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PurposeExpresses GFP tagged MLF2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 230986 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMLF2
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SpeciesH. sapiens (human)
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Entrez GeneMLF2 (a.k.a. NTN4)
- Promoter T7
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Tags
/ Fusion Proteins
- GFP (C terminal on insert)
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MLF2-GFP-3xFLAG was a gift from Christian Schlieker (Addgene plasmid # 230986 ; http://n2t.net/addgene:230986 ; RRID:Addgene_230986) -
For your References section:
Atypical nuclear envelope condensates linked to neurological disorders reveal nucleoporin-directed chaperone activities. Prophet SM, Rampello AJ, Niescier RF, Gentile JE, Mallik S, Koleske AJ, Schlieker C. Nat Cell Biol. 2022 Nov;24(11):1630-1641. doi: 10.1038/s41556-022-01001-y. Epub 2022 Oct 27. 10.1038/s41556-022-01001-y PubMed 36302970