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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 23098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-sport2
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4473
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemouse Gcn5 mRNA
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Alt nameKat2a
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SpeciesM. musculus (mouse)
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Insert Size (bp)3000
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Mutationmissing 50 bp upstream of ATG start site.
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GenBank IDNM_020004
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Entrez GeneKat2a (a.k.a. 1110051E14Rik, Gcn5, Gcn5l2, mmGCN5)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn I (unknown if destroyed)
- 3′ cloning site Apa I (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-sport2-mGCN5 FU, FLAG Tagged was a gift from Sharon Dent (Addgene plasmid # 23098 ; http://n2t.net/addgene:23098 ; RRID:Addgene_23098) -
For your References section:
Regulation of E2F1 activity by acetylation. Martinez-Balbas MA, Bauer UM, Nielsen SJ, Brehm A, Kouzarides T. EMBO J. 2000 Feb 15. 19(4):662-71. 10.1093/emboj/19.4.662 PubMed 10675335
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/87/08/0f323b20-af63-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)