pCMV-sport2-mGCN5 FU, FLAG Tagged
(Plasmid #23098)

• Depositing Lab: Sharon Dent
• Publication: Martinez-Balbas et al EMBO J. 2000 Feb 15. 19(4):662-71.

Backbone

• Vector backbone: pCMV-sport2
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4473
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mouse Gcn5 mRNA
• Alt name: Kat2a
• Species: M. musculus (mouse)
• Insert Size (bp): 3000
• Mutation: missing 50 bp upstream of ATG start site.
• GenBank ID: NM_020004
• Entrez Gene: Kat2a (a.k.a. 1110051E14Rik, Gcn5, Gcn5l2, mmGCN5)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (unknown if destroyed)
• 3′ cloning site: Apa I (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry