CROPseq-tRFP
(Plasmid
#230938)
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Purpose(Empty Backbone) Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 230938 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCROPseq-Guide-Puro
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Backbone manufacturerChristoph Bock, Addgene plasmid #86708
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Modifications to backboneRemoval of puromycin resistance and addition of TagRFP
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Vector typeLentiviral
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Tag
/ Fusion Protein
- TagRFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CROPseq-tRFP was a gift from Hyejung Won (Addgene plasmid # 230938 ; http://n2t.net/addgene:230938 ; RRID:Addgene_230938) -
For your References section:
Massively parallel reporter assay investigates shared genetic variants of eight psychiatric disorders. Lee S, McAfee JC, Lee J, Gomez A, Ledford AT, Clarke D, Min H, Gerstein MB, Boyle AP, Sullivan PF, Beltran AS, Won H. Cell. 2025 Jan 22:S0092-8674(24)01435-1. doi: 10.1016/j.cell.2024.12.022. 10.1016/j.cell.2024.12.022 PubMed 39848247