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pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR
(Plasmid #230935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-U6-gRNA-UbC-eGFP-P2A-Bsr
  • Backbone manufacturer
    Charles Gersbach (Addgene plasmid # 83925)
  • Modifications to backbone
    modified eGFP to TagBFP
  • Vector type
    Lentiviral, CRISPR
  • Promoter hU6
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • TagBFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.04.611293 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR was a gift from Charles Gersbach (Addgene plasmid # 230935 ; http://n2t.net/addgene:230935 ; RRID:Addgene_230935)

  • For your References section:

    Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER. Liu S, Hamilton MC, Cowart T, Barrera A, Bounds LR, Nelson AC, Dornbaum SF, Riley JW, Doty RW, Allen AS, Crawford GE, Majoros WH, Gersbach CA. Cell Genom. 2025 Feb 4:100766. doi: 10.1016/j.xgen.2025.100766. 10.1016/j.xgen.2025.100766 PubMed 39914388
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