pAAVtri-CMV-eGFP
(Plasmid #230932)

• Purpose: pAAVtri transgene plasmid is used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.
• Depositing Lab: Qizhao Wang
• Publication: AAVone: A Cost-Effective, Single-Plasmid Solution for Efficient AAV Production with Reduced DNA Impurities (unpublished)

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• Serotype: Select serotype for details See details about
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Backbone

• Vector backbone: pAAVtri
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP
• Species: Synthetic
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pAAVtri-CMV-eGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry