pCMV-Tag2B Flag BimEL(TD)
(Plasmid
#23092)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23092 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Tag2B
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBim EL (TD)
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Alt nameBim
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SpeciesM. musculus (mouse)
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Insert Size (bp)590
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MutationChanged Threonine 112 to Aspartate
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Entrez GeneBcl2l11 (a.k.a. 1500006F24Rik, Bim, Bod, bcl2-L-11)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer cmv F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Tag2B Flag BimEL(TD) was a gift from Roger Davis (Addgene plasmid # 23092 ; http://n2t.net/addgene:23092 ; RRID:Addgene_23092) -
For your References section:
Multisite phosphorylation regulates Bim stability and apoptotic activity. Hubner A, Barrett T, Flavell RA, Davis RJ. Mol Cell. 2008 May 23. 30(4):415-25. 10.1016/j.molcel.2008.03.025 PubMed 18498746